Figure 2

Geometry of the microfluidic device and details of domain and cell mesh. Left: two channels (gray) are separated by collagen I gel (cyan). Pressure and chemical gradients are established between inlet (purple) and outlet (orange) boundaries. A box-like domain (right) is taken from the central part of the gel to simulate the mechanical analysis and the cell migration. This domain is discretized with voxels of 3 μm, some of them considered cell-voxels and forming an initially spherical shape of about 30 μm of diameter embedded in the ECM to perform the mechanical and migration simulation.